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. 2016 Dec 5;16:287. doi: 10.1186/s12866-016-0904-3

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Preparation of CHAP. a Sephadex G-100 gelatin separation of the extract from chicken blood. b Antibacterial activity detection of elution of Sephadex G-100 gelatin by agarose diffusion assay against Escherichia coli ATCC25922. c Tricine–SDS-PAGE of the interested elution and the band was around 3.3 KDa (arrow)