Table 3.
Primers of the flavonoid biosynthesis pathway genes and housekeeping gene used for qRT-PCR.
| Target gene | Forward sequence (5′–3′) | Reverse sequence (5′–3′) | GenBank | AGI number |
|---|---|---|---|---|
| BnTT4 | GACTACTACTTCCGCATCACCAACAG | GCCTAGCTTAGGGACTTCAACAACC | AF076335 | AT5G13930 |
| BnTT5 | CTTCCTCGGTGGCGCAGGTG | ACACAGTTCTCCGTTACTTTCTCTGA | EU402417 | AT3G55120 |
| BnTT6 | TGGGTGAAAGTGACGGAGGAGT | TGGTTCCAGGGTCAGTGTGACG | DQ513329 | AT3G51240 |
| BnTT7 | GCCATAGCCCGTGACCCGGA | GCTTCTCCGGCGTAACTCCTCC | DQ324379 | AT5G07990 |
| BnTT3 | AGACCGTGTGCGTAACCGGC | AGGATCGCGAACAGTGGCACG | DQ767950 | AT5G42800 |
| BnTT18 | GGCTTAGAGCCTGACCGTCTAGAGAA | TGAGCTTCCACGCCAAGTGCT | GQ120562 | AT4G22880 |
| BnBAN | GGACTTGTGATGACCGAAGAAAACTG | ATGTAGCGACCAGAAGCTGTTTCTTT | FJ938339 | AT1G61720 |
| BnTT12 | GCTCCACAGAGACATACGAGCCG | ACGGTGACGAAGCTGAGCATGTA | EU818785 | AT3G59030 |
| BnTT19 | ACATCTTCTTCGTCAGCCATTTGGTCA | GGTCCACGATGGCTCGGTGC | AB117793 | AT5G17220 |
| BnTT10 | GCGACTGTGCCAAGAAACGGT | CCCCACGTGAGATGTCTATCAAAGTG | HM805059 | AT5G48100 |
| BnAHA10 | ACCCATTGCCATGCCCACTGT | GCTCGGCCTGCAAGCAACAA | NM_101587 | AT1G17260 |
| BnTT2 | AGCTGGTCTCAAGAGGTGTGGCA | AGCCTCCCAGCTATCAACGACC | DQ778647 | AT5G35550 |
| BnTT8 | GGCTGAAGAGGCTGCGTCGG | GTGCTGTGCAAGCCCTCGCT | EU192027 | AT4G09820 |
| BnTTG1 | TCCTCCGGCGACTTCCTCCG | GCTGCGTCTCCACCACGGAC | EF175930 | AT5G24520 |
| BnTT16 | TGCTCACATCGGTCTCATCGTCT | GCTCGTGTGGAGGAATGGAGGC | EU192028 | AT5G23260 |
| BnTTG2 | AAACCTAAAGCAAAGCTTGTCTCCCA | ACTTCCTTTGACTTGCTTCTGTCCGT | FJ012168 | AT2G37260 |
| BnTT1 | TCGCTACAACAATCTTCAGATGCACA | TCCTGCACCCTTCAACGCAGC | AF190298 | AT1G34790 |
| BnTT15 | ACAAAATGACGGGACAGTGGAAGTT | GGCTGCACATCGCCTCGAGTT | BT005834 | AT1G43620 |
| BnACTIN7 | TGGGTTTGCTGGTGACGAT | TGCCTAGGACGACCAACAATACT | EV116054 | AT5g09810 |
| BnUBC21 | CCTCTGCAGCCTCCTCAAGT | CATATCTCCCCTGTCTTGAAATGC | EV086936 | AT5g25760 |
Primers for amplifying partial sequences of flavonoid biosynthesis genes were designed from conserved nucleotide regions identified by multiple alignments of sequences.