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Images of an asymmetric GUV (Inner leaflet: DOPC – Outer leaflet: DMPC) aspirated into a micropipette with increasing suction pressure (i.e. membrane tension) from image (a) to (d). The aspiration length at each suction pressure was recorded and compared to that in image (a), which has the lowest suction pressure, to calculate the change in aspiration length (ΔL). The measurement data of this GUV is shown in Figure 4.
