Table 2.
Primer sequences. Allele-specific primer sequences for FCGR2A and -3A genes. The polymorphic sites are shown in bold. To increase PCR specificity, a mismatch was inserted at penultimate position of the respective allele-specific primer. GAPDH served as internal control.
Gene | Primer | Polymorphism | Sequence (5′–3′) |
---|---|---|---|
FCGR2A | forward | 131H | ATGGAAAATCCCAGAAATTCTCACA |
forward | 131R | ATGGAAAATCCCAGAAATTCTCACG | |
reverse | H131R | AATGACCACAGCCACAATGA | |
FCGR3A | forward | V158F | GGTCACATATTTACAGAATGGCAAACG |
reverse | 158V | CAGTCTCTGAAGACACATTTTTACTCCCATC | |
reverse | 158F | CAGTCTCTGAAGACACATTTTTACTCCCATA | |
GAPDH | forward | ACTAGGCGCTCACTGTTCTCTC | |
reverse | GGAGTAGGGACCTCCTGTTTCT |