Figure 3.

Generation of Th1 CD4⁺ T-cell lines against BCR framework region peptides from normal donors. (A) IFNγ ELISA assay of donor T cells stimulated with PBMC which have known HLA DRB1 alleles as antigen-presenting cells (APCs), pulsed or nonpulsed with BCR framework region peptides. (B) Intracellular cytokine staining of BCR framework region-specific CD4⁺ T cells stimulated by normal donor APCs, pulsed or nonpulsed with peptides. (C) Blocking of IFNγ production by BCR framework region-specific CD4⁺ T cells by HLA antibodies. (D) Identification of 15-mer minimal T-cell epitopes by BCR framework region-specific CD4⁺ T cells by IFNγ ELISA assay. Data are shown as mean value of triplicate wells ± SEM and are representative of three individual experiments.

Figure 3.

(Continued)