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. 2016 Sep 9;5(11):e1232219. doi: 10.1080/2162402X.2016.1232219

Figure 2.

Figure 2.

Sequential PRR stimulation of BMDC enhances cytokine mRNA expression and NFκB and MAP kinase signaling. (A) Il-6, Il-12a, and Il-12b mRNA expression 4 and 8 h after the second stimulation in BMDC stimulated as in Fig. 1. Mean + SEM of four to eight independent experiments are shown. (B) Immunoblot analysis of p65 and MAPK in BMDC stimulated with combinations of poly(I:C) and R848, at 0, 20 and 40 min, after the second stimulation. Individual blots are indicated by rectangles and are representative of three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001, and ****p < 0.0001, compared to the sequential PRR stimulation; one-way ANOVA, Dunetts post-test.