Figure 3.

Sequential PRR stimulation of BMDC increases activation of effector T cells and NK cells. (A) Expression of CD69 on CD4⁺ and CD8⁺ splenocytes added to sequentially stimulated BMDC 1.5 h after the second stimulation and cocultured for 24 h. Representative histograms and MFI + SEM of quadruples are shown. (B) Expression of CD69 on purified NK cells co-cultured with BMDC as in (A). (C) IFNγ levels in supernatants of splenocytes and purified NK cells co-cultured with BMDC as in (A) and (B). (D) Experimental protocol for the stimulation of BMDC and co-culture with splenocytes or purified NK cells. MFI + SEM of quadruples are shown. Data are representative of two independent experiments.