Figure 2.

LMW-HA enhances interstitial-lymphatic flow in C57BL/6 mice. (A) A total of 20 µL of high-molecular-weight FITC-dextran (8 mg/mL) was intradermally co-injected with LMW-HA (5 µg), HMW-HA (5 µg), or medium (control) into the tails of mice (female, 8 weeks old). Five mice were used in each experimental group. Photographs were taken under LED illumination every 30 min until 120 min had elapsed. A representative sample of five mice is shown. FITC-dextran fluorescence near the injection site was measured. (B) Fluorescence intensities at the initial time (0 min) were set to 100%, and the fluorescence values at the other time points were calculated as the percentage of the value at 0 min. Bars represent the mean ± SD, n = 5, *p < 0.05, ANOVA.