Figure 8.

N1 TANs present antitumor properties. (A) Ly6G⁺ cells isolated from AB12 tumors from control (N2) and SM16-treated animals (N1) (n = 5–7 for each group) were co-cultured with AB12-luciferase cells at ratios of 1:5, 1:10 or 1:20 (tumor cells:TANs). The graph summarizes the percentage of tumor cell killing ± SEM at each ratio of co-culture at 24 h (n = 4–6). (B) In a Modified Winn Assay, AB12 tumor cells were injected together with N1 or N2 TANs (n = 6 in each group) and tumor growth was measured regularly. N1 TANs significantly impaired tumor growth and tumor size remained significantly low relative to N2 until the end of the experiment (day 7). (C) AB12 tumor cells injected alone had an intermediate growth compare to co-injection with either N1 or N2 TANs.