Table 1.
Summary of the relative changes in pathways and gene groups between naive bone marrow neutrophils (BMN), non-treated tumor neutrophils (N2) and following TGFβ inhibition with SM16 (N1).
| Neutrophil Function | BMN | N2 | N1 | ||
|---|---|---|---|---|---|
| Structural genes | Cytoskeleton | + | − | + | |
| Actin polymerization | ++ | + | ++ | ||
| Response to stress | TLRs | + | +/− | + | |
| Respiratory burst | ++ | +/− | +/− | ||
| Granule Proteins | Primary | ++ | − | − | |
| Secondary | ++ | − | − | ||
| Tertiary | +++ | ++ | ++ | ||
| Vesicle formation | Receptor-mediated phagocytosis | +++ | + | +++ | |
| Endocytosis | +/− | +/− | +/− | ||
| Apoptosis | Whole group | + | + | + | |
| NFkB anti-apoptotic | ++ | +/− | +/− | ||
| Inflammatory response | Whole group | − | ++ | +++ | |
| Antigen processing and presentation | − | ++ | +++ | ||
| Cytokine activity | − | ++ | ++ | ||
| Chemokine activity | − | +++ | +++ | ||
Pathways and gene groups were evaluated by the Genomica software, KEGG, and manually from the literature. The data of each neutrophils function evaluated for each population of neutrophils is presented.
(−) Most genes in the pathway/group were at background levels.
(+/−) Some genes of the pathway/group were upregulated and other downregulated.
(+) A related pathway/group was upregulated (Genomica), or some (>10 %) of the genes in the group were upregulated (manually).
(++) A related pathway/group was upregulated (Genomica), and/or a significant portion (>30 %) of the genes in the group were upregulated (manually).
(+++) A prominent upregulation of genes in the group/pathway (>50 %) was noted.