. 2016 Nov 2;49(Pt 6):2015–2020. doi: 10.1107/S1600576716016514

Table 1. Experimental (fitted using GRASP) structural parameters derived from the results of in situ SEC-SANS analysis of the BioRAD calibration standard in 100% D₂O buffer.

MW: molecular weight; Extinc. coef.: extinction coefficient; Abs.: absorbance; I _(Q=0): scattering intensity extrapolated at Q = 0; R _g: radius of gyration. For comparison, literature data are extracted from references 1: Ianeselli et al. (2010a ▸,b ▸); 2: Doster et al. (2003 ▸); 3: Goldenberg & Argyle (2014 ▸); 4: Fujisawa & Kato (2002 ▸).

	Concentration determination			Experimental values (GRASP)		Literature data
Parameter	MW (g mol⁻¹)	Extinc. coef. [(cm mg ml⁻¹)⁻¹]	Abs. 280 nm (a.u.)	I _(Q=0) (cm⁻¹)	R _g (Å)	R _g (Å)
Ovalbumin	44000	0.7	0.272	0.142 ± 0.001	24.0 ± 0.4	23–24¹
Myoglobin	17000	0.82	0.433	0.0401 ± 0.0005	14.0 ± 0.4	14.8 ± 0.2²
						13.9³
						17.5 ± 0.1⁴

Table 1. Experimental (fitted using GRASP) structural parameters derived from the results of in situ SEC-SANS analysis of the BioRAD calibration standard in 100% D2O buffer.

Table 1. Experimental (fitted using GRASP) structural parameters derived from the results of in situ SEC-SANS analysis of the BioRAD calibration standard in 100% D₂O buffer.