Table 2.

Specimen support geometries for particular applications

Mode	Grid	Foil	Film
Negative stain	400 mesh	none	50–100 Å am-C
Diagnostic cryo	300 mesh	1.2/1.3 μm	none / 20 Å am-C
Medium-resolution (≥3.5 Å) cryo	300 mesh	1.2/1.3 μm	none / 20 Å am-C
High-resolution cryo (<3.5 Å) >400 kDa	300 mesh	1.2/1.3 μm	none / 20 Å am-C
High-resolution cryo (<3.5 Å) <400 kDa	300 mesh	1.2/1.3 μm	none / graphene
Very high-resolution cryo (<2.8 Å)	300 mesh	0.6/1.0 μm	none / graphene
Cryo-tomography: cellular (>30 Å)	200 mesh	2.0/2.0 μm *	none
Cryo-tomography: high-resolution sub-tomogram avg. (<15 Å)	300 mesh	1.2/1.3 μm	none / 20 Å am-C

^*Larger holes may be used for particularly large specimens.