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. Author manuscript; available in PMC: 2016 Dec 6.
Published in final edited form as: Plant J. 2014 May 21;79(4):679–692. doi: 10.1111/tpj.12503

Figure 5. Novel approaches based on metabolomics to the discovery of regulatory mechanisms for herbivory-induced changes in phenolamide metabolism.

Figure 5

(a) High-throughput non-targeted metabolite profiling of herbivory-induced changes in a large collection of RNAi transgenic lines reveals regulators of metabolite accumulation. Processed data can be classified using hierarchical clustering and clusters of m/z signals of interest screened across the library of transgenic lines. The jasmonate regulation of N’,N”-caffeoyl,feruloyspermidine is provided as an example. (b) Natural variation in W+OS-induced levels of N-caffeoylputrescine and of an unknown spermidine-based phenolamide in 176 natural accessions of N. attenuata positively correlate with natural variation in the OS-induced JA-Ile bursts.