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. Author manuscript; available in PMC: 2016 Dec 6.
Published in final edited form as: Clin Immunol. 2009 Jan 22;131(2):240–253. doi: 10.1016/j.clim.2008.12.006

Figure 2.

Figure 2

Figure 2

(a) Representative HC sample for Treg gating strategy. PBMC (peripheral blood mononuclear cells) were stained for LiveDead Stain (Invitrogen), CD4+, CD25hi, CD127lo. They were then gated by Live/Dead, then SSC and FSC for the lymphocyte gate, then CD4+, then CD127lo/CD25hi cells. This gating strategy was used to gate for all Treg. Using this Treg gating, additional markers were then assessed as in panel b. (b) Representative HC sample for gating strategies for the additional markers co stained with the Treg population (panel a).