Fig 5. Clonality analysis of each tumor from patient A7.

Clonality shared among the primary tumor and matched metastases in patient A7 (A) and the representative evolutionary tree (B) colored by subclone identity based on the clonality plots in panel A, with the width representative of the percentage of the clone within that tumor. Clone 1 was established in the primary tumor and maintained through metastatic spread in every tumor. Clone 2 was present in the liver, kidney, and rib and at a low frequency in the lung, while clones 3 and 5 were shared by the liver and kidney metastases. Clone 6 was present in the rib and a low frequency in the lung metastases. Brain and lung metastases shared clone 4. Four metastases had a private clone not shared with any other tumor: clone 7 specific to the lung, clone 8 specific to the kidney, clone 9 specific to the liver, and clone 10 private to the brain.