Fig 1. Identification of novel lncRNAs in glial tumors and normal brain RNA-seq samples.

(A) Overview of analysis pipeline for identifying novel lncRNAs and determining their associations with clinically relevant phenotypes. (B) Cumulative distribution function plot of CPAT scores demonstrates that the majority of novel transcripts are predicted to not code for proteins (CPAT score < 0.5242). (C) Metagene plot of H3K4me3 ChIP-seq data from U87 cell samples shows enrichment in promoters of protein-coding genes and novel lncRNAs but not in a randomized genomic control. GBM, glioblastoma multiforme; LGG, lower grade glioma; lncRNA, long noncoding RNA; RNA-seq, RNA sequencing.