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. 2016 Dec 6;12(12):e1006474. doi: 10.1371/journal.pgen.1006474

Fig 3. TF binding motif analysis of stage specific enhancers and identification of super-enhancer associated genes during brown adipogenesis.

Fig 3

(A) and (B) Differentiation stage-specific enhancers (defined as H3K27ac positive and H3K4me3 negative regions) are also marked by H3K4me1 and associate with genes reflecting their developmental stages. (C) and (D) TF binding motif analysis of stage specific enhancers with HOMER. “Site” indicates the bound TF(s), “Gene” indicates the corresponding gene coding for (one of) the TF(s). Expression of genes was determined using RNA-seq data. Sites with enriched motifs and correspondingly expressed genes were shown in the list. In differentiated BAs and WAs, the binding motifs for PPARγ, RXR and C/EBP were enriched, whereas the SIX1 motif was only enriched in BAs at late stage. (E) Genes associated with super-enhancers (SEs) in mature BAs and WAs. A subset of SE associated genes is shared between BAs and WAs, including established adipogenic markers such as Cebpa and Fabp4, whereas known brown and brite selective genes are specifically associated with SEs in BAs. (F) The Rreb1 gene was significantly up-regulated during brown adipogenesis and is associated with a SE in BAs.