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. 2016 Dec 6;12(12):e1006474. doi: 10.1371/journal.pgen.1006474

Fig 4. BMP7 triggers a transcriptional response during BA lineage commitment largely dependent on p38 signaling.

Fig 4

(A) Heatmap of coding genes transiently up-regulated upon BMP7 treatment. C3H10T1/2 cells were treated with or without BMP7 for 3 days before differentiation was induced at d0 (BA). Global gene expression profiles were generated by RNA-seq at the indicated time points. 3T3-L1 cells were also included in the analysis (WA). (B) Five members of the Sox gene family were transiently induced by BMP7 treatment. (C) Gene ontology analysis of the 89 BMP7 induced coding genes from (A). (D) and (E) Blocking the p38 signaling pathway by inhibitors PD169316 (PD) or SB202190 (SB) abolished the BMP7 induced transcriptional activation of all five Sox genes (D) as well as 27 other BMP7 target genes (E). Values represent the average of three biological replicates. Error bars indicate standard deviation. p-values: (paired student’s t-test) * <0.05; ** <0.01.