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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: In Vitro Cell Dev Biol Anim. 2016 Jan 7;52(4):497–506. doi: 10.1007/s11626-015-9997-5

Figure 3. Cell number and blastocyst diameter of embryos.

Figure 3

cultivated in CTRL condition or in coculture with iMSC or iMEF for 3 days (~114 hours after mating). (A) Total cell number per embryo, assessed by observation of serial confocal optical sections of embryos stained with DAPI. (B) Blastocyst diameter. Both experiments were performed in same structures and the total number was n=61 for CTRL, n=50 for iMSC and n=56 for iMEF groups. Group abbreviations: control condition (CTRL); cocultured with inactivated mesenchymal cells (iMSC); cocultured with inactivated murine embryonic fibroblasts (iMEF). *P<0.05 and ***P<0.001