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. Author manuscript; available in PMC: 2017 Apr 1.
Published in final edited form as: In Vitro Cell Dev Biol Anim. 2016 Jan 7;52(4):497–506. doi: 10.1007/s11626-015-9997-5

Figure 6. Immunostaining for inner cell mass and trophoblast in embryos cultivated in CTRL condition or with cells for 3 days (~114 hours after mating).

Figure 6

The antibody anti-Oct 4 (in red) was used to differentiate inner cell mass from trophoblast by exclusion since all cells were counterstained with DAPI (in blue) as represented in the sequential images (A-A″) CTRL, (B-B″) iMSC and (C-C″) iMEF groups. (D) The absolute number of Oct 4-positive cells per embryo and (E) the percentage of Oct 4-positive cells. (F) Total number of trophoblast and (G) percentage of trophoblast. n=27 for CTRL, n=19 for iMSC and n=25 for iMEF groups. Group abbreviations: control condition (CTRL); cocultured with inactivated mesenchymal cells (iMSC); cocultured with inactivated murine embryonic fibroblasts (iMEF). *P<0.05 and **P<0.01