Fig. 3.

Representative QuasAr2 fluorescence spike plots (from a single neuron per exposure) show block and recovery following a (a) 0.52, (b) 0.96, and (c) $1.05\mathrm{J}/{\mathrm{cm}}^2$ SILP. Blue light was pulsed at 10 Hz. SILP exposure at $t=0\mathrm{s}$. Recovery of blue light stimulated AP is demonstrated at (d) 8 to 10 s for the $0.52\mathrm{J}/{\mathrm{cm}}^2$ SILP [same cell as (a)], (e) 11.5 to 13.5 s for the $0.96\mathrm{J}/{\mathrm{cm}}^2$ SILP [same cell as (b)], and (f) 300 (5 min) to 302 s for the $1.05\mathrm{J}/{\mathrm{cm}}^2$ SILP [same cell as (c)]. Blue dots represent blue light exposure. (g) The average length of SILP-induced AP block increased with increased exposure ($n=9$ to 33 cells/data point; $\text{mean}\pm \mathrm{SE}$). (h) The average time of recovery to 100% firing efficiency increased with SILP exposure ($n=9$ to 22 cells, $\text{mean}\pm \mathrm{SE}$).