Fig. 4.

(a) Representative QuasAr2 fluorescence spike plot demonstrating consistent AP generation prior to SILPs, reduced AP activity during the repeated SILPs ($0.52\mathrm{J}/{\mathrm{cm}}^2$; 3 Hz), and following the SILP exposure. Blue light (5-ms exposure) was pulsed at 10 Hz. Red dots represent SILPs. (b) Enlarged display of $-2$ to 5 s data in (a), showing the consistent APs prior to the SILP exposure ($t<0$); and the block during exposure ($t>0$). Blue dots represent blue light exposure. (c) Enlarged display of the 35 to 40 s data in (a) showing recovery of consistent AP. (d) Average SPS for neurons prior to SILP (blue led pulsed at 10 Hz), during 30 s of $0.52\mathrm{J}/{\mathrm{cm}}^2$ SILP pulsed at 3 ($n=6$ cells), 5 ($n=6$ cells), or 7 ($n=6$ cells) Hz, and following SILP. $\text{Mean}\pm \mathrm{SE}$, $n=6$ to 9 cells. *$p<0.05$ versus pre-SILP. ^$p<0.05$ versus 3 Hz SILP. †$p<0.05$ versus 5 Hz SILP. (e) Average SPS for neurons prior to SILP (blue light pulsed at 10 Hz), during 30 s of $0.79\mathrm{J}/{\mathrm{cm}}^2$ SILP pulsed at 1 ($n=5$ cells), 3 ($n=7$ cells), or 5 ($n=6$ cells) Hz, and following SILP. $\text{Mean}\pm \mathrm{SE}$, *$p<0.05$ versus pre-SILP. ^$p<0.05$ versus infrared 1 Hz.