Figure 4. Chemogenetic inhibition of medial prefrontal cortex (mPFC) neurons reverses the effect of REM sleep loss on highly-palatable-foods consumption.
(A) Drawings of superimposed adeno-associated viruses (AAV) injection sites in the mPFC of mice used for baseline (n = 3; left panel) or wire-mesh-grid device (WMGD) experiments [n = 4 in the propylene glycol (PG), middle panel, or ivermectin (IVM), right panel, treated group]. (B) Grams of sucrose or fat consumed over a 25–48 hr period under baseline (nine wild-type mice treated with saline and three AAV-injected mice treated with PG) and WMGD [AAV-injected mice treated with PG (n = 4) or IVM (n = 4)] conditions. *p < 0.05, **p < 0.01 and ***p < 0.001 indicates significant differences between mice groups (one-tailed).
Figure 4—figure supplement 1. Control experiments for highly palatable foods consumption in mice.
(A) Grams of sucrose or fat consumed over a 25–48 hr period for wild-type mice treated with saline (n = 9) or AAV-injected mice treated with PG (n = 3) compared to AAV-injected mice treated with IVM (n = 4). (B) Grams of sucrose or fat consumed over a 25–48 hr period correlated with baseline body weight in grams for all mice.