Figure 3. Full training during the sleep phase with anisomycin causes long-term memory.

Experiments were performed on A. californica. (A) Training animals until they stop responding during the inactive phase 10 min after treatment with anisomycin (N = 7) leads to memory when animals are tested 24 hr later, as shown by a decrease in the time stop responding (p=0.05, t = 2.49, df = 6; two-tailed paired t-test). (B) Training animals until they stop responding during the inactive phase 10 min after treatment with ASW (N = 5) did not lead to long-term memory, as shown by a lack of savings when animals were tested 24 hr later (p=0.3, t = 1.12, df = 4; two-tailed paired t-test). (C) Anisomycin treatment alone during the inactive phase (N = 7) does not produce memory 24 hr later (p=0.74, t = 0.34, df = 16 – data after anisomycin treatment were compared to data for naïve animals trained during the day in Figure 1B2, which were trained along with these animals.

DOI: http://dx.doi.org/10.7554/eLife.17769.007