Figure 5. Nep4 is expressed in glial cells and neurons in the central nervous system.
nep4 expression was visualized using a reporter construct that drives nuclear GFP (nGFP) expression in a nep4-specific manner (nep4 > nGFP, green). Reversed polarity protein was labeled with a monospecific antibody (α-Repo, red). (A–C) Optical projections of third instar larval whole brain-ventral nerve cord complexes. Scale bars: 100 µm; dorsal view, anterior up. Boxes indicate areas of higher magnification, as depicted in (D–F) and (G–I). Within the brain, nep4 expression was strongest in the central brain (A, dashed line) and in lamina cells (A, brackets), while only few nep4-positive medulla cells were observed (A, bar). Within the ventral nerve cord, nep4 was expressed in numerous cells along all segments. (D–I) Optical projections of third instar larval brain hemisphere (D–F) and ventral nerve cord (G–I). Scale bars: 20 µm; dorsal view, anterior up, midline to right. nep4 expression colocalized extensively with anti-Repo staining.