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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2016 Jun 9;36(8):1517–1524. doi: 10.1161/ATVBAHA.116.307281

Figure 1. Analysis of maternal anti-HPA-1a derived from −ICH (n=18) and +ICH (n=18) in an antigen capture assay.

Figure 1

HPA-1aa platelets (upper panel) or endothelial cells (bottom panel) were incubated with anti-HPA-1a sera and moabs against aIIbb3 (a), avb3 (c) and b3 (b, d). After cell lysis, the moab-(aIIbb3/avb3/b3)-anti-HPA-1a trimolecular complex was immobilized on microtiter wells coated with anti-mouse IgG. Binding of anti-HPA-1a antibodies to platelet-derived aIIbb3, aIIbb3 + avb3 and endothelial-derived avb3 was detected with enzyme labelled anti-human IgG. Statistical analysis was performed by Mann-Whitney U Test.