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Schematic representation of the MS2-TRAP methodology, including the plasmids required (‘Plasmids’), the fusion RNA and protein expressed from the plasmids (‘Chimeric Molecules’) and the resulting RNP complex (‘Complex in Affinity pulldown/IP’). After pulldown or immunoprecipitation using the appropriate conjugated beads, RNAs and proteins bound to the chimeric transcript can be identified using are methodologies listed (gray box).
