Figure 1.

The depletion of extracellular nucleotides reduces Pam₃CSK₄-induced neutrophil migration in vivo. Mouse air pouches were raised on the back of female CD-1^® mice by subcutaneous injection of sterile air. Six days later, mice were split in three groups and were injected in the air pouches with 1 mL PBS (control) or synthetic tripalmitoylated lipopeptide Pam₃CSK₄ (Pam, 10 ng), with or without apyrase (Apy, 2 U) in PBS, respectively. The cells (largely neutrophils) accumulated in the pouches were collected 1 and 4 h later and analyzed as described in the Materials and methods. Data show mean+SEM of seven or eight mice per group. ^*p = 0.03.