Figure 6.

Downregulation of either P2Y₂ or P2Y₆ receptor impairs IL-8 release in THP-1 cells. THP-1 cells (3 × 10⁶/5 mL) were infected with lentivirus carrying scrambled shRNA or shRNA targeting human P2Y₂ or P2Y₆ mRNA as described in the Materials and methods. (A) Knockdown quantification of P2Y₂ and P2Y₆ mRNA levels by qRT-PCR. (B) The shRNA treated cells were stimulated with Pam₃CSK₄ as before and IL-8 secretion measured. Data show mean+SEM of four different experiments, each performed in duplicate or triplicate. One hundred percent was set with the media of Pam₃CSK₄-treated THP-1 and represents 18±4 ng/mL secreted IL-8. ^*p<0.05; ^**p<0.01; ^***p<0.001.