Fig 2.

PFOS exposure increases the frequency of anomalous pancreas morphologies during development. (A) Islet morphology was examined in Tg(insulin-GFP) transgenic fish at 48, 96, and 168 hpf after subchronic PFOS exposure beginning at 3 hpf. Islets were screened for fragmentation, hollowness, and severely stunted growth (shown in B at 20× magnification). Numbers presented are the percent of embryos/larvae with variant islets. Italicized numbers are the number of embryos/larvae sampled, cumulative across several study replicates. Fewer than 5% of embryos and larvae were severely deformed at the time of sampling, and were excluded from pancreas imaging. The distribution of islet morphologies are shown in pie charts under each respective time point, indicating a difference in the types of variants observed throughout development. No significant temporal differences were observed. The position of the islet within the zebrafish is shown (B, left). Asterisks (*) indicate a difference between designated treatment group and the controls (p<0.05); n=30-45 embryos at 48 hpf; n=20-25 eleutheroembryos at 96 hpf; n=24-29 larvae at 168 hpf