Fig 5.
Embryonic PFOS exposure alters pancreas endocrine gene expression. RNA was isolated from embryos collected at 48 and 96 hpf, following subchronic PFOS exposure since 3 hpf. Expression of insa (A), gcga (B), pdx1 (C), sst2 (D), and ghrl (E) was analyzed using qPCR and the ΔΔCT method. Bars represent the average fold change (relative to beta actin; shown on y-axis) and the control group, and stars represent a PFOS-associated statistically significant change of expression from the control group. Age of the embryos and eleutheroembryos is shown on the x-axis in hpf. Asterisks (*) indicate a difference between designated treatment group and the controls (p<0.05); n=7-9 samples of 9 pooled embryos at 48 hpf; n=4-5 samples of 5 pooled eleutheroembryos at 96 hpf