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. Author manuscript; available in PMC: 2017 May 7.
Published in final edited form as: Nat Struct Mol Biol. 2016 Nov 7;23(12):1092–1100. doi: 10.1038/nsmb.3319

Figure 2.

Figure 2

Telomerase preferentially incorporates 8-oxodGTP opposite rA. (a) and (d) Telomerase activity assays were conducted using 5 nM 32P-end labeled (TTAGGG)3 (a) or (GGTTAG)3 (d) primers. Reactions contained cellular dNTPs (lane 2) or increasing amounts (5, 50, 500 μM) of dGTP (lanes 3 – 5), 8-oxodGTP (lanes 6 – 8), dTTP (lanes 9 – 11, panel a) or dATP (lanes 9 – 11, panel d). Products were separated on denaturing gels. Numbers on the left show number of added repeats, and letters on right indicate template base. (b) and (e) The percent of total primers extended for each reaction. (c) and (f) The percent of primers terminated at each template position as a function of total radioactivity in the lane. Bars represent the mean ± sd from three independent reactions. * p < 0.05, ** p < 0.01, *** p < 0.001, two-tailed Student’s t test. N.D. = not determined. Uncropped gel images are shown in Supplemental Data Set 1.