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. Author manuscript; available in PMC: 2017 May 7.
Published in final edited form as: Nat Struct Mol Biol. 2016 Nov 7;23(12):1092–1100. doi: 10.1038/nsmb.3319

Figure 3. Cells with shortened telomeres are hypersensitive to oxidized nucleotides.

Figure 3

HeLa cell lines with very short telomeres (VST) or long telomeres (LT) were analyzed 3 days after transduction with lentivirus expressing a non-targeting shRNA (scr) or different shRNAs against MTH1 (sh4 and sh5). (a) Immunoblot with antibodies against MTH1 or actin. The percent MTH1 expression relative to the control was calculated. (b) Population doubling values are the mean ± s.d. from four independent experiments. * p<0.05, ** p<0.01, One-way ANOVA with Tukey’s honest significance difference. (c) Analysis for apoptosis by immunoblotting for caspase 3 activation. (d) Flow cytometry results of annexin V/PI staining. The numbers above the red box indicate percent of total annexin V-FITC positive cells (both early and late apoptosis). (e) Quantitation of percent annexin V-FITC positive cells. * p<0.05, ** p<0.01, two-tailed Student’s t test. Uncropped blot images are shown in Supplemental Data Set 1.