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. Author manuscript; available in PMC: 2017 Dec 5.
Published in final edited form as: Curr Biol. 2016 Nov 17;26(23):3230–3237. doi: 10.1016/j.cub.2016.09.053

Figure 2. Efficient secretory vesicle traffic requires Tpm1 function in Bni1-mediated actin cable assembly.

Figure 2

(A) Representative images of early log phase cells showing distribution of GFP-Sec4 (false colored). Scale bar, 3 μm. (B) Ratio of bud to mother GFP-Sec4 fluorescence. Each dot is the ratio quantified for one cell (n ≥ 40 cells per strain analyzed). Black line, Mean. Student’s T-test used to determine the indicated significant difference (p < 0.05) from wildtype (‘w’), appropriate tropomyosin single mutant (‘t’), or appropriate formin single mutant (‘f’). (C) Ratio of bud to mother GFP-Sec4 fluorescence plotted as a function of bud size (same data as in B). Lines fit by linear regression using standard equations in Prism 6.