Effect of LPL and FASN knockdown on cell proliferation (left panel) and apoptosis (middle panel) of SK-Hep1 (A) and MHCC97H (B) human HCC cell lines. Cells were treated with transfection reagent only (control), scramble siRNA, FASN siRNA, siLPL or siFASN+siLPL and collected for analysis after 48 hours treatment. Each bar represents mean ± SEM. Tukey–Kramer test: P < 0.05 a, vs control; b, vs scramble siRNA; c, vs LPL siRNA; d, vs FASN si RNA. (C) and (D): Western blotting analysis showing the decreased expression of FASN and/or LPL protein expression after siRNA treatment in SK-Hep1 (C) and MHCC97H (D) human HCC cell lines.