Table 1. Oligonucleotides and applications.
| Name | Oligonucleotide sequence | Application |
|---|---|---|
| FEN1 L209P F | 5′-CCAGGAATTCCACCCGAGCCGGATTCTGC-3′ | Mutagenesis |
| FEN1 L209P R | 5′-GCAGAATCCGGCTCGGGTGGAATTCCTGG-3′ | |
| FEN1-21-T | 5′-ATGCGATACAGTCCGATAGCT-3′ | FEN1 FEN activity assay |
| FEN1-40 | 5′-32P-CCATGAGCAACTACGATATGCGTACTAAGCCTAATCCGAC-3′ | |
| FEN1-A-F-G-40 | 5′-GTCGGATTAGGCTTAGTACGGCTATCGGACTGTATCGCAT-3′ | |
| FEN1-21-EXO | 5′-ATGCGATACAGTCCGATAGCC-3′ | FEN1 EXO activity assay |
| FEN1-19-EXO | 5′-32P-GTACTAAGCCTAATCCGAC-3′ | |
| FEN1-A-F-G-40 | 5′-GTCGGATTAGGCTTAGTACGGCTATCGGACTGTATCGCAT-3′ | |
| FEN1-10-GAP | 5′-TTGCTCATGG-3′ | |
| FEN1-20-GAP | 5′-ATGCGATACAGTCCGATAGC-3′ | FEN1 GAP activity assay |
| FEN1-40 | 5′-32P-CCATGAGCAACTACGATATGCGTACTAAGCCTAATCCGAC-3′ | |
| FEN1-A-F-G-40 | 5′-GTCGGATTAGGCTTAGTACGGCTATCGGACTGTATCGCAT-3′ | |
| FEN1-F | 5′-CTTACACGTTGACTACCTTFTTTGAGGCAAGAGTGGATCC-3′ | LP-BER assay |
| 3′-GAATGTGCAACTGATGGCAGAAACTCCGTTCTCACCTAGG-5′ |
Abbreviations: EXO, exonuclease; FEN1, flap endonuclease-1; GAP, gap endonuclease; LP-BER, long-patch base excision repair.