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. 2016 Jun 6;35(49):6330–6340. doi: 10.1038/onc.2016.164

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Copyright © 2016 Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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SUB1 is overexpressed in aggressive prostate cancer. (a) SUB1 gene expression from next-generation RNA sequencing (RPKM (log 2)) data from benign, prostate carcinoma (PCa) and metastatic prostate cancer (MET) tissues. (b) Expression of SUB1 in normal prostate, primary tumor and metastatic tumor samples from TCGA. (c) qPCR of SUB1 using RNA from benign, PCa and MET tissues. (d) SUB1 protein expression by immunoblot analysis of prostate tissue extracts using SUB1 antibody. β-Actin was used as a loading control. (e) Immunoblot analysis of SUB1 in prostate cancer cell lines. GAPDH was used as a loading control. (f) Immunohistochemical analysis of SUB1 in benign prostate epithelia (top), primary PCa (middle) and metastatic PCa (bottom).