Table I.
Glucosinolate profile of cyp79F1 and cyp79F2 mutants and cyp79F1 cyp79F2 double mutant
| Short-Chain Glucosinolates
|
Long-Chain Glucosinolates
|
Indole Glucosinolates
|
|||||||
|---|---|---|---|---|---|---|---|---|---|
| 3-msp | 5-msp | 3-mtp | 7-msh | 8-mso | 8-mto | i-3ym | 4mi-3ym | Nmi-3ym | |
| Rosette Leaves | |||||||||
| Wild type | 10.67 ± 0.92 | 0.24 ± 0.02 | 0.24 ± 0.02 | 0.05 ± 0.002 | 0.61 ± 0.04 | 0.06 ± 0.008 | 1.33 ± 0.06 | 0.25 ± 0.02 | 0.29 ± 0.06 |
| cyp79F1 | ND | ND | ND | 0.05 ± 0.006 | 1.66 ± 0.12 | 0.14 ± 0.009 | 2.14 ± 0.07 | 0.24 ± 0.01 | 0.013 ± 0.001 |
| cyp79F2 | 9.88 ± 0.85 | 0.22 ± 0.03 | 0.24 ± 0.02 | 0.05 ± 0.008 | 0.47 ± 0.05 | 0.05 ± 0.007 | 1.42 ± 0.13 | 0.25 ± 0.01 | 0.27 ± 0.04 |
| cyp79F1 cyp79F2 | ND | ND | ND | ND | ND | ND | 2.2 ± 0.08 | 0.24 ± 0.02 | 0.03 ± 0.02 |
| Root | |||||||||
| Wild type | 7.38 ± 0.69 | 0.24 ± 0.05 | 0.22 ± 0.01 | 0.02 ± 0.002 | 0.36 ± 0.02 | 0.12 ± 0.01 | 0.9 ± 0.06 | 0.24 ± 0.02 | 11.3 ± 0.87 |
| cyp79F1 | ND | ND | ND | 0.03 ± 0.004 | 0.58 ± 0.04 | 0.21 ± 0.02 | 0.73 ± 0.08 | 0.15 ± 0.01 | 7.97 ± 0.35 |
| cyp79F2 | 5.02 ± 0.37 | 0.32 ± 0.05 | 0.18 ± 0.02 | 0.01 ± 0.0002 | 0.17 ± 0.03 | 0.37 ± 0.02 | 0.79 ± 0.04 | 0.16 ± 0.007 | 13.3 ± 0.58 |
| cyp79F1 cyp79F2 | ND | ND | ND | ND | ND | ND | 0.44 ± 0.07 | 0.11 ± 0.03 | 6.05 ± 1.43 |
| Stem | |||||||||
| Wild type | 8.38 ± 0.22 | 0.1 ± 0.01 | 0.07 ± 0.02 | 0.04 ± 0.01 | 0.44 ± 0.10 | 0.005 ± 0.001 | 0.33 ± 0.01 | 0.06 ± 0.007 | 0.007 ± 0.001 |
| cyp79F1 | ND | ND | ND | 0.19 ± 0.01 | 5.58 ± 0.39 | 0.03 ± 0.004 | 2.15 ± 0.16 | 0.002 ± 0.0003 | 0.02 ± 0.005 |
| cyp79F2 | 14.29 ± 0.86 | 0.14 ± 0.03 | 0.13 ± 0.01 | 0.07 ± 0.01 | 0.44 ± 0.11 | 0.02 ± 0.001 | 0.47 ± 0.02 | 0.05 ± 0.01 | 0.01 ± 0.002 |
| cyp79F1 cyp79F2 | ND | ND | ND | ND | ND | ND | 2.12 ± 0.17 | 0.002 ± 0.0002 | 0.018 ± 0.002 |
| Flower | |||||||||
| Wild type | 33.02 ± 0.99 | 0.27 ± 0.03 | 0.93 ± 0.05 | 0.57 ± 0.03 | 7.76 ± 0.46 | 0.06 ± 0.007 | 1.32 ± 0.07 | 0.03 ± 0.005 | 0.009 ± 0.002 |
| cyp79F1 | ND | ND | ND | 0.41 ± 0.007 | 11.8 ± 0.31 | 0.18 ± 0.008 | 3.02 ± 0.08 | 0.04 ± 0.003 | 0.01 ± 0.002 |
| cyp79F2 | 28.36 ± 2.11 | 0.24 ± 0.02 | 1.17 ± 0.01 | 0.47 ± 0.008 | 5.47 ± 0.11 | 0.08 ± 0.01 | 1.72 ± 0.07 | 0.03 ± 0.001 | 0.02 ± 0.007 |
| cyp79F1 cyp79F2 | ND | ND | ND | ND | ND | ND | 2.77 ± 0.05 | 0.03 ± 0.001 | 0.01 ± 0.0006 |
3-msp, 3-methylsulfinylpropyl glucosinolate; 5-msp, 5-methylsulfinylpentyl glucosinolate; 3-mtp, 3-methylthiopropyl glucosinolate; 7-msh, 7-methylsulfinylheptyl glucosinolate; 8-mso, 8-methylsulfinyloctyl glucosinolate; 8-mto, 8-methylthiooctyl glucosinolate; i-3ym, indole-3-ylmethyl glucosinolate; 4mi-3ym, 4-methoxyidol-3-ylmethyl glucosinolate; Nmi-3ym, N-methoxyindol-3-ylmethyl glucosinolate.
ND, Not determined.