Figure 3.

The fate of assimilated ¹⁴C during continuing photosynthesis. Nine LL- or HL-grown wild-type (○) or ape2 (•) plants were placed in a sealed glass container in which was released 500 μL L^{−1 14}CO₂ (1,000 μL L⁻¹ for HL wild type). After 15 min photosynthesis under growth light conditions, the chamber was flushed through with air and three plants were removed either immediately or after a further 2 and 4 h, and metabolism was quenched by immediately freezing leaf tissue in ethanol. The data show the absolute ¹⁴C content of metabolite fractions expressed relative to rosette area, with correction for the unlabeled CO₂ present in the chamber (mean ± se, n = 3). Note the 2-fold difference between HL and LL in the scales for the y axes. Average recovery of label following fractionation of the ethanol-insoluble and ethanol-soluble components was 95% and 80%, respectively.