Table I.
HL
|
LL
|
|||
---|---|---|---|---|
Ws-2 | ape2 | Ws-2 | ape2 | |
Soluble | 61.6 ± 1.9 | 59.5 ± 1.5 | 54.3 ± 1.0 | 47.9 ± 1.3a |
Neutral sugars | 32.2 ± 1.6 | 22.2 ± 0.8a | 18.6 ± 0.9 | 10.9 ± 0.2a |
Fructose | 2.2 ± 0.2 | 0.8 ± 0.0a | 0.9 ± 0.0 | 0.3 ± 0.0a |
Glucose | 4.6 ± 0.3 | 3.2 ± 0.4b | 2.2 ± 0.1 | 1.2 ± 0.0a |
Sucrose | 24.9 ± 1.2 | 17.8 ± 0.7a | 15.3 ± 0.8 | 9.1 ± 0.3a |
Other | 0.6 ± 0.1 | 0.5 ± 0.1 | 0.2 ± 0.0 | 0.3 ± 0.1 |
Amino acids | 17.2 ± 2.0 | 23.9 ± 1.5b | 21.6 ± 0.9 | 23.5 ± 0.8 |
Organic acids | 10.5 ± 1.3 | 10.9 ± 0.5 | 12.0 ± 0.5 | 11.4 ± 0.6 |
Phosphoesters | 1.6 ± 0.2 | 2.5 ± 0.5 | 2.2 ± 0.1 | 2.0 ± 0.2 |
Insoluble | 38.4 ± 1.9 | 40.5 ± 1.5 | 45.7 ± 1.0 | 52.1 ± 1.3a |
Starch | 30.3 ± 2.1 | 31.9 ± 1.3 | 35.2 ± 1.2 | 41.7 ± 1.3a |
Protein | 3.1 ± 0.2 | 3.5 ± 0.1 | 4.0 ± 0.3 | 4.2 ± 0.2 |
Cell wall | 5.1 ± 0.3 | 5.1 ± 0.3 | 6.5 ± 0.3 | 6.2 ± 0.3 |
For each growth condition, six wild-type and six ape2 plants were placed side by side in a sealed glass container in which was released 500 μL L−1 14CO2. After 15 min photosynthesis under growth light conditions, the chamber was flushed through with air and metabolism was quenched by immediately freezing leaf tissue in ethanol. The distribution of 14C between metabolite fractions was determined. The data for each fraction are expressed as a percentage of total recovered label (mean ± se, n = 6). The recovery of label following fractionation averaged 97%.
Significant difference from the corresponding wild type, P < 0.005.
Significant difference from the corresponding wild type, P < 0.05.