Table II.
Carotenoid composition and NADP-MDH activation state
| HL
|
LL
|
|||
|---|---|---|---|---|
| Ws-2 | ape2 | Ws-2 | ape2 | |
| Percentage total carotenoids | ||||
| Lutein | 44.3 ± 0.9 | 41.7 ± 0.5 | 46.1 ± 0.1 | 48.4 ± 1.3 |
| β-Carotene | 27.3 ± 1.6 | 28.5 ± 0.7 | 30.0 ± 0.5 | 24.1 ± 2.5 |
| Neoxanthin | 7.6 ± 0.3 | 7.8 ± 0.3 | 9.2 ± 0.2 | 10.9 ± 0.5 |
| Violaxanthin | 14.9 ± 0.8 | 9.9 ± 0.7 | 14.1 ± 0.5 | 15.6 ± 1.3 |
| Antheraxanthin | 4.4 ± 0.2 | 5.9 ± 0.4 | 0.6 ± 0.0 | 1.0 ± 0.4 |
| Zeaxanthin | 1.5 ± 0.2 | 6.1 ± 0.9 | 0.0 ± 0.0 | 0.0 ± 0.0 |
| Xanthophyll DES (%) | 17.8 ± 1.8 | 41.0 ± 3.2 | 2.1 ± 0.1 | 3.0 ± 1.2 |
| NADP-MDH activation state (%) | 53.5 ± 1.7 | 70.0 ± 1.8 | 53.3 ± 1.7 | 56.6 ± 1.9 |
Leaves were taken from plants under growth conditions 2 h into the photoperiod and were immediately frozen. They were subsequently used either for pigment extraction and analysis by reverse-phase HPLC or in assays of NADP-MDH activation state. For comparison, the NADP-MDH activation states of LL and HL wild-type plants transferred to saturating light levels for 30 min were 75 ± 1 and 69 ± 3 respectively, while in leaves darkened for 30 min it was 0.3 ± 4.3. All data are mean ± se, n = 4 to 5.