Figure 3.

Localization of the single-domain Str AtStr14 and AtStr18 in tobacco leaf epidermal and guard cells. Fusion constructs of AtStr14 and AtStr18 with the GFP encoding sequence were transformed in N. tabacum leaf cells by particle gun bombardment and were analyzed by fluorescence microscopy after overnight incubation. A, Bright field image of an epidermal N. tabacum cell. B, In the same cell shown in A, the fluorescence of the AtStr14/pGFP-N fusion protein was collected with the band pass filter (BP 450–490) for excitation and with the long pass filter (filter LP 520) for emission. C, Single merged image of a guard cell for AtStr14. D, Bright field image of a guard cell. E, In the same guard cell shown in D, the fluorescence was collected as described in B. All scale bars represent 10 μm.