Table I.
Protein Name | Base No. | Primer Pairs | Restriction Site | Fusion Vector |
---|---|---|---|---|
AtStr1woPS | 963 | P194 5′-cca tgg ctt cta ctg gag ttg a-3′ | NcoI | pGFP-N |
P195 5′-cca gat ctt gaa gaa gat tca ac-3′ | BglII | pGFP-C | ||
AtStr1wPS | 600 | P192 5′-cca tgg cct cga ccc ttt tct-3′ | NcoI | pGFP-N |
P193 5′-gga tcc cca tct tgg tag acc t-3′ | BamHI | |||
AtStr2woPS | 954 | P153 5′-cca tgg ctt ctt ctg gat ctg a-3′ | NcoI | pGFP-N |
P154 5′-gga tcc tga aga aga acc cac t-3′ | BamHI | pGFP-C | ||
AtStr2wPS | 1,098 | P155 5′-cca tgg cac gag gag aat ctg-3′ | NcoI | pGFP-N |
P154 5′-gga tcc tga aga aga acc cac t-3′ | BamHI | |||
AtStr14 | 711 | P138 5′-cca tgg ctt cac tta ctt caa-3′ | NcoI | pGFP-N |
P189 5′-cag atc tgt ctt ctt caa ttg ttt c-3′ | BglII | |||
AtStr15 | 546 | P132 5′-cca tgg aaa cca ctg ctt tta ac-3′ | NcoI | pGPF-N |
P150 5′-aga tct ctc ttc tac cgg cag c-3′ | BglII | pGFP-C | ||
AtStr16 | 360 | P134 5′-cca tgg acg agg aga gca gag t-3′ | NcoI | pGFP-N |
P151 5′-aga tct ttg aag aag aag gag acg-3′ | BglII | |||
AtStr18 | 408 | P136 5′-cca tgg ctc aat caa tct cct cc-3′ | NcoI | pGFP-N |
P152 5′-aga tct att agc aga tgg ctc ctc-3′ | BglII | pGFP-C |
The name of the fusion construct, the length of the PCR amplified DNA fragment, the primer pairs designed with the respective restriction sites, and the respective vector used for the fusions are listed. More information on the respective members of the multiprotein family is available at the Web site http://arabidopsis.org/info/genefamily/STR_genefamily.html.