Table II.
Localization of sulfurtransferases
| No. aa
|
Localization Prediction
|
||||
|---|---|---|---|---|---|
| Name | Gene ID | TP/total | PSORT | TargetP | Exp. |
| AtStr1 | At1g79230 | 58/379 | Mit matrix space (0.814) | cTP - 0.666 | Mit |
| microbody (peroxisome) (0.579) | mTP - 0.319 | ||||
| AtStr2 | At1g16460 | 48/366 | Chl stroma (0.607) | cTP - 0.233 | Cyt |
| Cyt (0.450) | SP - 0.165 | ||||
| AtStr14 | At4g27700 | 34/237 | Chl stroma (0.950) | cTP - 0.816 | Chl |
| Chl thylakoid membrane (0.800) | mTP - 0.258 | ||||
| AtStr15 | At4g35770 | 63/182 | Chl stroma (0.837) | cTP - 0.822 | Chl |
| Mit matrix space (0.598) | mTP - 0.067 | ||||
| AtStr16 | At5g66040 | 79a/120 | Cyt (0.450) | SP - 0.136 | Chl |
| Mit matrix space (0.360) | mTP - 0.108 | ||||
| AtStr17 | At2g17850 | 28a/150 | Nucleus (0.760) | SP - 0.136 | n.d. |
| microbody (peroxisome) (0.364) | mTP - 0.074 | ||||
| AtStr18 | At5g66170 | 10a/136 | Chl stroma (0.889) | cTP - 0.237 | Cyt |
| Chl thylakoid membrane (0.555) | SP - 0.120 | ||||
The protein name, gene identification, and the number of amino acids for six selected Str are summarized. PSORT and TargetP were used for the localization prediction, and SignalP V2.0 was used for the cleavage site prediction (http://www.expasy.ch/tools). Numbers in brackets give the certainty of prediction in PSORT; for the TargetP program, the respective probabilities are given; only the two highest scores of both programs are given. The results of the experiments done are summarized. aa, amino acids; Chl/c, chloroplast; Cyt, cytoplasm; exp., experimental; ID, identification; Mit/m, mitochondrial; n.d., not determined; SP, secretory pathway; TP, targeting peptide.
The prediction probability of the targeting peptide is low.