FIG 3 .

In vitro determination of the glyceraldehyde-3-P dehydrogenase activity in Pseudomonas putida KT2440 and its Δcra mutant. (A) Biochemical sequence proposed for the processing of glyceraldehyde-3-P. Enzymes involved in these conversions are shown beside the reaction that they catalyze. Note that the first biochemical step is catalyzed by the glyceraldehyde-3-P dehydrogenases (Gap-1 and Gap-2) and the two other isoforms of these enzymes, encoded by PP_0665 and PP_3443. All the reactions are conventionally written in the catabolic direction, and the wide shaded arrows indicate the connection of this series of biochemical reactions with the rest of the central carbohydrate metabolic pathways. (B) In vitro quantitation of the total specific (Sp) activity of glyceraldehyde-3-P dehydrogenase in P. putida KT2440 and its isogenic Δcra derivative determined in cells grown on glucose or fructose and using different redox cofactors as indicated. Each bar represents the mean value of the corresponding enzymatic activity ± standard deviation of duplicate measurements from at least three independent experiments. WT, wild-type strain.