Fig. 3.

Labeling of different peptides mapped to the same protein have similar kinetics. As examples, the traces in the plot (A) indicate exponential fits to peptide data for two different proteins with varying degradation rates, ACTB and AHNAK. The data was collected from MS2 TMT reporter tags of SILAC labeled precursors. The boxplots (B) provide a global analysis of variance in rate measurements between peptides belonging to the same protein. The box shows the median and interquartile range (IQR) of the coefficient of variations (CV) of degradation rates for peptides encompassing a single protein for all methods. The error bar represents the entire range of CVs excluding outliers (>1.5 IQR). The white lines and numbers represent mean values. The red circles indicate the CV among all measured peptide degradation rates for each method. The analysis was limited to proteins shared between all analyses.