Table II. ENO1 PTMs found in PC3 cells but not in purified ENO1 or PC3-DR cells.

Peptide fragment with PTMs (position)a	PTM	PC3 ENO1	PC3-DR ENO1	Purified ENO1
AAVPSGASTGIYEALELR (33–50)	T-phospho; E-methyl	Yes	No	No
DNDKTRYMGK (51–60)	D-methyl; T-phospho	Yes	No	No
TRYMGK (55–60)	Y-glycosyl	Yes	No	No
YMGKGVSKAVEHINK (57–71)	K-acetylation	Yes	No	No
GVSKAVEHINKTIAPALVSK (61–80)	K,H-methyl	Yes	No	No
LNVTEQEKIDKLMIEMDGTENK (82–103)	D-methyl	Yes	No	No
GVPLYR (127–132)	Y-phospho	Yes	No	No
IGAEVYHNLK (184–193)	Y-phospho; H-acetyl; K-methyl	Yes	No	No
YGKDATNVGDEGGFAPNILENK (200–221)	Y-phospho; K-methyl	Yes	No	No
DATNVGDEGGFAPNILENK (203–221)	T-phospho	Yes	No	No
EGLELLK (222–228)	K-acetyl	Yes	No	No
TAIGKAGYTDK (229–239)	T-phospho; K,D-methyl	Yes	No	No
VVIGMDVAASEFFR (240–253)	E-methyl	Yes	No	No
SGKYDLDFK (254–262)	K-acetyl	Yes	No	No
FTASAGIQVVGDDLTVTNPK (307–326)	Q,N-methyl; T-phospho	Yes	No	No
VNQIGSVTESLQACK (344–358)	Q-methyl	Yes	No	No
HRSGETEDTFIADLVVGLCTGQIK (371–394)	D-methyl	Yes	No	No
YNQLLR (407–412)	Q-methyl	Yes	No	No
LAKYNQLLR (404–412)	N-methyl	Yes	No	No
IEEELGSKAK (413–422)	K-acetyl	Yes	No	No

^a Position of the initial and final peptide residue in ENO1 protein sequence.