Table I. SRM and DTM concentrations (fmol/μg) and CVs for target peptides.
| Peptide | Protein name | Gene symbol | SRM Conca | SRM CVa | DTM Concb | DTM CVb | DTM/SRM Avg Concc | DTM/SRM CVc |
|---|---|---|---|---|---|---|---|---|
| R.TTILQSTGK.N | Parafibromin | CDC73 | 0.36 | 0.19 | 0.96 | 0.19 | 0.66 | 0.64 |
| R.IEDILEVIEK.E | Kynureninase isoform b | KYNU | 0.76 | 0.03 | 1.49 | 0.09 | 1.12 | 0.46 |
| R.SAPLEIGLQR.S | Parafibromin | CDC73 | 0.83 | 0.09 | 2.15 | 0.11 | 1.49 | 0.63 |
| R.LSASTASELSPK.S | MAP7 domain-containing protein 1 | MAP7D1 | 2.04 | 0.16 | 1.28 | 0.22 | 1.66 | 0.32 |
| R.EIFSVLDLMK.V | T-complex protein 11-like protein 1 | TCP11L1 | 1.28 | 0.02 | 2.66 | 0.08 | 1.97 | 0.50 |
| K.TYLEEELDKWAK.I | Kynureninase isoform b | KYNU | 4.34 | 0.11 | 2.09 | 0.12 | 3.21 | 0.49 |
| R.GLATFCLDKDALR.D | Membrane-associated progesterone receptor component 2 | PGRMC2 | 2.70 | 0.13 | 4.44 | 0.10 | 3.57 | 0.35 |
| R.TPETLLPFAEAEAFLKK.A | MAP7 domain-containing protein 1 | MAP7D1 | 4.56 | 0.03 | 2.75 | 0.19 | 3.65 | 0.35 |
| K.ETLLSFLLPGHTR.L | T-complex protein 11-like protein 1 | TCP11L1 | 2.65 | 0.03 | 6.47 | 0.08 | 4.56 | 0.59 |
| R.CLLETLALAPHEEYIQR.H | Transmembrane and TPR repeat-containing protein 3 | TMTC3 | 4.05 | 0.04 | 9.61 | 0.11 | 6.83 | 0.58 |
| R.DFTPAELR.R | Membrane-associated progesterone receptor component 1 | PGRMC1 | 13.05 | 0.03 | 10.23 | 0.11 | 11.64 | 0.17 |
| K.TIQEVAGYVLIALNTVER.I | Epidermal growth factor receptor isoform a precursor | EGFR | 12.82 | 0.36 | 10.49 | 0.10 | 11.66 | 0.14 |
| K.ALPILEELLR.Y | Transmembrane and TPR repeat-containing protein 3 | TMTC3 | 10.31 | 0.09 | 16.40 | 0.07 | 13.36 | 0.32 |
| R.GLGAGAGAGEESPATSLPR.M | Membrane-associated progesterone receptor component 2 | PGRMC2 | 12.25 | 0.16 | 17.91 | 0.06 | 15.08 | 0.27 |
| R.VFDKDGNGYISAAELR.H | Calmodulin | CALM2 | 22.14 | 0.04 | 29.39 | 0.07 | 25.76 | 0.20 |
| K.FYGPEGPYGVFAGR.D | Membrane-associated progesterone receptor component 1 | PGRMC1 | 24.44 | 0.08 | 32.01 | 0.06 | 28.22 | 0.19 |
| R.DVVICPDASLEDAKK.E | Protein DJ-1 | PARK7 | 30.83 | 0.17 | 32.64 | 0.06 | 31.73 | 0.04 |
| K.LTQLGTFEDHFLSLQR.M | Epidermal growth factor receptor isoform a precursor | EGRF | NA | NA | 32.42 | 0.06 | 32.42 | NA |
| K.VTVAGLAGKDPVQCSR.D | Protein DJ-1 | PARK7 | 40.87 | 0.08 | 43.06 | 0.07 | 41.97 | 0.04 |
| K.EAFSLFDKDGDGTITTK.E | Calmodulin | CALM2 | 113.46 | 0.06 | 126.28 | 0.03 | 119.87 | 0.08 |
The concentration (fmol/μg) and CV values observed for each peptide by aSRM and bDTM analyses.
c The average concentration and error for both analyses were also computed to understand differences.