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. 2016 Nov 2;6:36355. doi: 10.1038/srep36355

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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IFN-γ ELISPOT was performed on either 250,000 PBMC or 250,000 CD4-depleted PBMC (CD8/others) using single peptide pools consisting of 15-mers overlapping by 11 amino acids (final concentration of each peptide pool is 5μg/ml) spanning each of the three antigens contained in the AERAS-402 vaccine. All determinations are performed in duplicate. Solid line: Ag85A, dashed line: Ag85B, dotted line: TB10.4. For the CD8 ELISPOT assay (CD8/others), CD8⁺ T cells were negatively selected from PBMC using a combination of CD4 and CD56 magnetic beads. For the PBMC ELISPOT, unfractionated PBMC were used as the source of responding T cells and largely consist of CD4⁺ T cells. Participant numbers are designated over the graphs with the two participants receiving placebo noted in parentheses. Participant 02309 received only the first dose of AERAS-402 vaccine (day 0), due to an abnormal hematocrit prior to the planned day 28 dose.