Figure 2. Intracellular Cytokine Staining Response to the AERAS-402 Vaccine.

PBMC and leukapheresis specimens from Study Days −84, −14, 28, 56, and 98 were thawed, rested overnight, and stimulated for 5–7 hours with DMSO (negative control), SEB (positive control), or peptide pools corresponding to the vaccine antigens Ag85A, Ag85B, or TB10.4. Specimens were then stained for viability, phenotypic markers, and intracellular cytokine expression and evaluated by flow cytometry. The gating strategy is shown (a). The total DMSO-subtracted cytokine response for CD4⁺ and CD8⁺ T cells following stimulation with Ag85A (b,c), Ag85B (d,e), and TB10.4 (f,g). Each circle represents the response from a single participant. Bars represent the median response for each group. Data is shown for participants immunized with BCG on Study Day −84 and placebo (black circles) and participants vaccinated with BCG on Study Day −84 followed by vaccination with AERAS-402 on Study Days 0 and 28 (3 × 10¹⁰ vp; red circles).